By T. Leeteera, P. Asanithi, P. Limsuwan and S. Limsuwan
Assembly of silk fibroin protein as a 3-dimentional hydrogel was demonstrated by taking advantage of the difference in phase transition behavior of the protein and water. Effect of Ca2+ ions remained in the fibroin protein solution after the preparation of protein solution on the formation of hydrogel was investigated as a function of dialysis time, including 1, 2 and 3 days. The swelling behavior of the hydrogel was reported. The information from this study may be useful for medical applications of silk fibroin hydrogel.